Influenza A virus (H3N2), Purified (ATCC® VR-544PQ)

Classification: Orthomyxoviridae, Alphainfluenzavirus  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 CEID50/mL

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Classification Orthomyxoviridae, Alphainfluenzavirus
Deposited As Influenza A2
Agent Influenza A virus (H3N2), Purified
Strain A/Hong Kong/8/68
Applications Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Testing disinfectants
Testing virucides
This strain is recommended by ATCC for use in the tests described in Federal Specifications A-A-1438, A-A-1439, A-A-1440, A-A-1441, and A-A-1443 where only the taxon is specified.
Cross-reacts in HAI test with A2 strains at low dilutions.
Influenza Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 CEID50/mL
Storage Conditions -70°C or colder
Comments ATCC® VR-544PQ™ is a high-titer, purified, live virus suspended in 1X PBS + 0.1% Sodium azide. This product was prepared from ATCC® VR-544™ via purification through sucrose gradient centrifugation.

This strain is recommended by ATCC® for use in the tests described in Federal Specifications A-A-1438, A-A-1439, A-A-1440, A-A-1441, and A-A-1443 where only the taxon is specified. Cross-reacts in HAI tests with A2 strains at low dilutions.
Effect on Host
Hemagglutinates chicken RBC
Recommended Host
SPF chicken embryonated eggs, 10-11 days
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Dilute virus in D-PBS prior to inoculation.

Incubation: 2-3 days with humidity and non-CO2 incubator

Effect on Host
Hemagglutinates chicken RBC
Name of Depositor MT Coleman; ATCC
Special Collection NCRR Contract
Source Patient, Hong Kong, 1968
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

. . Morbid. & Mortality Weekly Report (NCDC) 17(33): 305, 1968.

. . WHO Weekly Epid. Record 43: 401, 1968.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation