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Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 9027-MINI-PACK)

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Strain Designations: R. Hugh 813  /  Type Strain: no  /  Biosafety Level: 2

Verified By Whole-genome Sequencing
Strain Designations R. Hugh 813
Application ATCC® 9027-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 9027™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! .

Assay of antimicrobial preservatives
Efficacy testing
Media testing
Preparatory test control
Quality control strain
Sterility testing
Produces rhamnolipid surfactant
Pharmaceutical and Personal Care
Isolation Outer ear infection
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
6 ready-to-use vials of ATCC® 9027™ in glycerol stock
Storage Conditions -70°C or colder, store upright. Storage at -20°C is acceptable for up to 1 year.
Preceptrol® no
Type Strain no
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor CP Hegarty
Special Collection ATCC® Minis
Isolation Outer ear infection
References

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

Zhang Y, Miller RM. Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl. Environ. Microbiol. 58: 3276-3282, 1992. PubMed: 1444363

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 1997

U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Jones CB, Platt JH. Propofol composition containing edetate. US Patent 5,714,520 dated Feb 3 1998

Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.

Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.

Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.

Preservatives-effectiveness test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.12.

Ophthalmic optics--Contact lens care products--Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14729:2001.

Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14730:2000.

Applied Biosystems, MicroSeq PHL Panel.

Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria. London, UK:British Standards Institution;British Standard BS ISO 21149:2006.

Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa. London, UK:British Standards Institution;British Standard BS ISO 22717:2006.

U.S. Pharmacopeia General Chapters: <61> Microbial Examination of Nonsterile Products: Microbial Enumeration Tests. Rockville, MD USP34-NF29, 2011

U.S. Pharmacopeia General Chapters:<62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Rockville, MD USP34-NF29, 2011

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.