To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at For Technical questions please contact Thank you.

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >

Kluyveromyces marxianus (EC Hansen) van der Walt (ATCC® 8554)

Alternate State: Candida kefyr (Beijerinck) van Uden et Buckley, anamorph  /  Strain Designations: [15, type E, ATCC 34439, CBS 5795, CCRC 21480, DSM 5418, NCYC 1426, NRRL Y-1109, UCD 55-61, VTTC-81112]  /  Product Format: freeze-dried

Permits and Restrictions

View Permits

Deposited As Kluyveromyces fragilis (Jorgensen) van der Walt
Classification Fungi, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Kluyveromyces
Strain Designations [15, type E, ATCC 34439, CBS 5795, CCRC 21480, DSM 5418, NCYC 1426, NRRL Y-1109, UCD 55-61, VTTC-81112]
Alternate State Candida kefyr (Beijerinck) van Uden et Buckley, anamorph
Assay of trichothecenes
Bacterial resistance testing adhesives
Degrades glucose D-glucose
Degrades whey permeate
Ferments pentoses
Fungus resistance testing adhesives
Produces acetoin dehydrogenase diacetyl reductase
Produces beta-galactosidase beta-D-galactosidase
Produces biomass
Produces butanediol dehydrogenase acetoin reductase
Produces ethyl alcohol ethanol
Produces glycerol glycerin, glycerine
Produces lactase
Produces single-cell protein SCP
Produces biomass on whey permeate of cheddar cheese
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Morphology After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains.  Growth is butyrous, glossy, cream-colored to brown.
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence


D1D2 region of the 26S ribosomal RNA gene


Morphology After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains.  Growth is butyrous, glossy, cream-colored to brown.
Name of Depositor Lederle Labs.
Chain of Custody
ATCC <-- Lederle Labs. <-- N.C. Laffer 15, type E
Meadowgold creamery, USA

Crisan EV, Sorensen SG. Production and utilization of lactase. US Patent 4,007,283 dated Feb 8 1977

Schwarz JG, Hang YD. Study of acetoin reductase from Kluyveromyces marxianus. Lett. Appl. Microbiol. 16: 14-16, 1993.

Maleszka R, Schneider H. Fermentation of D-xylose, xylitol, and D-xylulose by yeasts. Can. J. Microbiol. 28: 360-363, 1982. PubMed: 6211222

Madhyastha MS, et al. Optimization of yeast bioassay for trichothecene mycotoxins. J. Food Prot. 57: 490-495, 1994.

chen KC, et al. Search method for the optimal medium for the production of lactase by Kluyveromyces fragilis. Enzyme Microb. Technol. 14: 659-664, 1992.

ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.

Mahoney RR, et al. Selection of strain, growth conditions, and extraction procedures for optimum production of lactase from Kluyveromyces fragilis. J. Dairy Sci. 58: 1620-1629, 1975. PubMed: 1194465

Mahoney RR. Number and nature of the sulphydryl groups of beta-galactosidase from Kluyveromyces fragilis. J. Food Biochem. 4: 189-199, 1980.

DeLaney RA, et al. Composition of Saccharomyces fragilis biomass grown on lactose permeate. J. Sci. Food Agric. 26: 1177-1186, 1975.

Inchaurrondo VA, et al. Yeast growth and þ-galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994.

Schwarz JG, Hang YD. Kluyveromyces marxianus: a potential source of diacetyl reductase. World J. Microbiol. Biotechnol. 10: 385-387, 1994.

Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994.

Freer SN, Detroy RW. Characterization of cellobiose fermentations to ethanol by yeasts. Biotechnol. Bioeng. 25: 541-557, 1983.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation