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Escherichia coli (Migula) Castellani and Chalmers (ATCC® 700891)

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Strain Designations: HS(pFamp)R  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations HS(pFamp)R
Control Culture
Bacteriophage host
Coliphages in water
Control strain
Enumeration of F+ RNA coliphages
Water testing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antibiotic Resistance
Resistant to ampicillin
Resistant to streptomycin
This strain contains the Famp plasmid.
Medium ATCC® Medium 2553: Tryptic Soy w/ 150 mcg/ml of Ampicillin and 150 mcg/ml Streptomycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor M Sobsey
Special Collection ATCC
Chain of Custody
ATCC <-- M Sobsey <-- VJ Cabelli <-- JB Kaper

Debartolomeis J, Cabelli VJ. Evaluation of an Escherichia coli host strain for enumeration of F male-specific bacteriophages. Appl. Environ. Microbiol. 57: 1301-1305, 1991.

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001

Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR.

USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001

9224 C: Male-specific coliphage assay using Escherichia coli Famp. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;.

Fast Phage Test. Charm.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019