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Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299)

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Strain Designations: NJ-3  /  Type Strain: no  /  Biosafety Level: 2

Permits and Restrictions

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Verified By Whole-genome Sequencing
Strain Designations NJ-3
Quality control strain used for high-level aminoglycoside resistance testing
Susceptibility disc testing
Susceptibility testing
Peritoneal fluid, St. Louis, MO
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genotype vanB ant(6)-I aac(6') aph(2"")
Type Strain no
Antibiotic Resistance
Low-level vancomycin-resistant, VanB
Resistant to Vancomycin. Sensitive to Teicoplanin.
Presence of vanB confirmed by PCR
Medium ATCC® Medium 2174: Brain heart infusion medium with vancomycin 4 mcg/ml
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype vanB ant(6)-I aac(6') aph(2"")
Name of Depositor FC Tenover
Chain of Custody
ATCC <-- FC Tenover <-- J Hoppe-Bauer
Peritoneal fluid, St. Louis, MO

Tenover FC, et al. Ability of clinical laboratories to detect antimicrobial agent- resistant enterococci [published erratum appears in J. Clin. Microbiol. 31: 3081, 1993]. J. Clin. Microbiol. 31: 1695-1699, 1993. PubMed: 8349745

Swenson JM, et al. Molecular characterization and multilaboratory evaluation of Enterococcus faecalis ATCC 51299 for quality control of screening tests for vancomycin and high-level aminoglycoside resistance in enterococci. J. Clin. Microbiol. 33: 3019-3021, 1995. PubMed: 8576364

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

BD Phoenix Gram Positive AST PMIC/ID QC Set. BD Becton Dickinson; Phoenix

MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens.

Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

VITEK 2 AST-GP QC Set. bioMerieux.

MicroScan Synergies plus Gram Positive QC Set. Dade/Siemens.

BD GeneOhm VanR QC Set. BD Becton Dickinson.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Patel R, et al. Multiplex PCR Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci. J. Clin. Microbiol. 35(3): 703-707, 1997. PubMed: 9041416

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019