Bacillus atrophaeus Nakamura (ATCC® 51189)

Strain Designations: NCTC 10073 [Camp Detrick, DSM 2277, NCIB 8649, NRCB-467]  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Bacillus subtilis (Ehrenberg) Cohn
Strain Designations NCTC 10073 [Camp Detrick, DSM 2277, NCIB 8649, NRCB-467]
Media testing
Sterility testing
Ethylene oxide sterilization control
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Type Strain no
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 30.0°C
Name of Depositor NCTC
Chain of Custody
ATCC <<--NCTC<<--J.C. Kelsey (Bacillus globigii) <<--- C.R. Phillips

Beeby MM, Whitehouse CE. A bacterial spore test piece for the control of ethylene oxide sterilization. J. Appl. Bacteriol. 28: 349-360, 1965. PubMed: 4954908

Kelsey JC. Use of gaseous antimicrobial agents with special reference to ethylene oxide. J. Appl. Bacteriol. 30: 92-100, 1967. PubMed: 6045112

Fritze D, Pukall R. Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus. Int. J. Syst. Evol. Microbiol. 51: 35-37, 2001. PubMed: 11211269

Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003.

Sterilization of health care products--Biological indicators--Part 2: Biological indicators for ethylene oxide sterilization. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-2:1994.

Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.

Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation