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Lactobacillus casei (Orla-Jensen) Hansen and Lessel (ATCC® 393)

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Strain Designations: 03 [7, IAM 12473, Orland L-323, R.P. Tittsler 303]  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Lactobacillus casei subsp. casei (Orla-Jensen) Hansen and Lessel
Verified By Whole-genome Sequencing
Strain Designations 03 [7, IAM 12473, Orland L-323, R.P. Tittsler 303]
Produces acetoin acetylmethylcarbinol
Produces diacetyl
Quality control strain
Dairy products
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain yes
Alpha-acetolactate biosynthesis
Medium ATCC® Medium 416: Lactobacilli MRS Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor GJ Hucker
Chain of Custody
ATCC <-- GJ Hucker <-- S Orla-Jensen 7 (Streptobacterium casei)
Dairy products

Hansen PA, Lessel EF. Lactobacillus casei (Orla-Jensen) comb. nov.. Int. J. Syst. Bacteriol. 21: 69-71, 1971.

Collins MD, et al. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and lactobacillus rhamnosus sp. nov., comb. nov.. Int. J. Syst. Bacteriol. 39: 105-108, 1989.

Wayne LG. Actions of the Judicial Commission of the International Committee on Systematic Bacteriology on requests for opinions published between January 1985 and July 1993. Int. J. Syst. Bacteriol. 44: 177-178, 1994.

Alpert CA, Siebers U. The lac operon of Lactobacillus casei contains lacT, a gene coding for a protein of the Bg1G family of transcriptional antiterminators. J. Bacteriol. 179: 1555-1562, 1997. PubMed: 9045813

Auvray F, et al. Plasmid integration in a wide range of bacteria mediated by the integrase of Lactobacillus delbrueckii bacteriophage mv4. J. Bacteriol. 179: 1837-1845, 1997. PubMed: 9068626

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Barnes AL, Keenan TW. Biosynthesis of alpha-acetolacetate and its converison of diacetyl and acetion in cell-free extracts of Lactobacillus casei. Can. J. Microbiol. 18: 479-485, 1972. PubMed: 4623970

AN MicroPlate QC Set. Biolog.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References

Nucleotide (GenBank) : Z80834 L.casei lacT gene.

Nucleotide (GenBank) : AJ006018 Lactobacillus casei priA gene.

Nucleotide (GenBank) : M23928 L.casei small subunit ribosomal RNA.

Nucleotide (GenBank) : D16551 Lactobacillus casei DNA for 16S rRNA.

Nucleotide (GenBank) : AJ003194 Lactobacillus casei ccpA & tnp genes.

Nucleotide (GenBank) : Z75480 L.casei rrn operon, 23S-5S rRNA spacer (long).

Nucleotide (GenBank) : Z75481 L.casei rrn operon, 23S-5S rRNA spacer (short).

Nucleotide (GenBank) : Z75479 L.casei rrn operon, 16S-23S rRNA spacer (short).

Nucleotide (GenBank) : AF129168 Lactobacillus casei sorbose operon, partial sequence.

Nucleotide (GenBank) : M76708 L.casei lactate dehydrogenase (ldh) gene, complete cds.

Nucleotide (GenBank) : Y18948 Lactobacillus casei hprK gene, lgt gene (partial) and 3 ORF's.

Nucleotide (GenBank) : AF385770 Lactobacillus casei 16S ribosomal RNA gene, partial sequence.

Nucleotide (GenBank) : Z75478 L.casei rrn operon, 16S-23S rRNA spacer (long), tRNA-Ile and tRNA-Ala genes.

Nucleotide (GenBank) : U28137 Lactobacillus casei general catabolite repressor Ccpa protein (ccpA) gene, complete cds.

Nucleotide (GenBank) : AJ251789 Lactobacillus casei bacteriophage A2 integration genes, lysis/lysogeny decision module and replication module genes.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Other Document
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019