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1.800.638.6597

Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. (ATCC^® 25416^™)

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Strain Designations: UCB 717 [717-ICPB 25, Ballard 717, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, HAMBI 1976, ICMP 5796, IFO 14074, JCM 5964, LMG 1222, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810] / Type Strain: yes / Biosafety Level: 2

Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC^® 25416^™

freeze-dried

For-Profit: $67.00 Non-Profit: $56.95

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Permits and Restrictions	View Permits
Deposited As	Pseudomonas cepacia (Burkholder) Palleroni and Holmes
Verified By	Whole-genome Sequencing
Strain Designations	UCB 717 [717-ICPB 25, Ballard 717, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, HAMBI 1976, ICMP 5796, IFO 14074, JCM 5964, LMG 1222, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810]
Application	Assay of antimicrobial preservatives Media testing Produces cepabactin Quality control strain Opportunistic pathogen research Respiratory research
Isolation	Plant-derived foodstuff, onion (Allium cepa), 1948
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section

Preceptrol®	yes
Type Strain	yes
Comments	This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified. Taxonomy Pyrimidine synthesis

Medium	ATCC^® Medium 3: Nutrient agar or nutrient broth
Growth Conditions	Temperature: 30°C Atmosphere: Aerobic

Name of Depositor	RW Ballard, WH Burkholder
Special Collection	ATCC
Chain of Custody	ATCC <-- RW Ballard, WH Burkholder
Isolation	Plant-derived foodstuff, onion (Allium cepa), 1948
References	Meyer JM, et al. Cepabactin from Pseudomonas cepacia, a new type of siderophore. J Gen Microbiol 135: 1479-1487, 1989. PubMed: 2533244 Gluzman Y, Ahrens B. SV40 early mutants that are defective for viral DNA synthesis but competent for transformation of cultured rat and simian cells. Virology 123: 78-92, 1982. PubMed: 6293195 ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA Ballard RW, et al. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J Gen Microbiol 60: 199-214, 1970. PubMed: 5488054 Palleroni NJ, Holmes B. Pseudomonas cepacia sp. nov., nom. rev. Int J Syst Bacteriol 31: 479-481, 1981. Validation list no. 45. Int. J. Syst. Bacteriol. 43: 398-399, 1993. Li K, West TP. Pyrimidine synthesis in Burkholderia cepacia ATCC 25416. Lett Appl Microbiol 21: 340-343, 1995. PubMed: 7576530 Yabuuchi E, et al. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. [published erratum appears in Microbiol Immunol 37: 335, 1995]. Microbiol Immunol 36: 1251-1275, 1992. PubMed: 1283774 Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Hubner A, Hendrickson W. A fusion promoter created by a new insertion sequence, IS1490, aciivates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in Burkholderia cepacia AC1100. J. Bacteriol. 179: 2717-2723, 1997. PubMed: 9098071 Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10. Applied Biosystems, MicroSeq PHL Panel.
Cross References	Nucleotide (GenBank) : M22518 P. cepacia 16S ribosomal RNA, complete cds. Nucleotide (GenBank) : U96927 Burkholderia cepacia 16S ribosomal RNA gene, partial sequence. Nucleotide (GenBank) : AJ293344 Burkholderia cepacia partial alkB gene for putative alkane 1-monooxygenase. Nucleotide (GenBank) : U85788 Burkholderia cepacia plasmid pPEC320 polygalacturonase (pehA) gene, complete cds. Nucleotide (GenBank) : L36602 Rhizobium meliloti (clone Rhizo70-1) heat shock protein 70 (hsp70) gene, complete cds. Nucleotide (GenBank) : L36603 Pseudomonas cepacia (clone Psudom70-1) heat shock protein 70 (hsp70) gene, complete cds. Nucleotide (GenBank) : U51235 Mycoplasma capricolum ClpB (clpB), partial cds, GrpE (grpE), and DnaK (dnaK) genes, complete cds, and DnaJ (dnaJ) gene, partial cds.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
FAQs	Accessing genome sequencing data Date Updated: 9/30/2019 Criteria for reference quality genomes Date Updated: 9/30/2019