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Bacillus cereus Frankland and Frankland (ATCC® 11778)

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Strain Designations: FDA strain PCI 213 [ATCC 9634, NCTC 10320, S.A. Waksman strain O]  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Bacillus mycoides Flugge
Verified By Whole-genome Sequencing
Strain Designations FDA strain PCI 213 [ATCC 9634, NCTC 10320, S.A. Waksman strain O]
Assay of chlortetracycline aureomycin
Assay of demeclocycline demethylchlortetracycline
Assay of doxycycline hydramycin
Assay of minocycline
Assay of oxytetracycline terramycin
Assay of tetracycline
Media testing
Quality control strain
Food testing
Quality control strain for BBL products
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor FDA

Appl. Microbiol. 17: 91, 1969.

Proc. Soc. Exp. Biol. Med. 55: 66-69, 1944.

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.

Demezas DH, Bell J. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. Syst. Appl. Microbiol. 18: 582-589, 1995.

Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 4986725

Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution;British Standard BS EN ISO 21871:2006.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References

Nucleotide (GenBank) : U02893 Bacillus cereus ATCC 11778 16S rRNA gene, partial sequence.

Nucleotide (GenBank) : AF238886 Bacillus cereus strain ATCC 11778 putative VrrB (vrrB) gene, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019