Human genomic DNA (HCC1143) (ATCC® CRL-2321D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1
Introduction

Cell Line Description:

HCC1143 is a poorly differentiated cell line that was initiated on August 30, 1994; it took 29 months to establish. HCC1143 was derived from an invasive ductal carcinoma with no lymph node metastases.

The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1143 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for  expression of estrogen receptor (ER) and progesterone receptor (PR). DNA isolated from an EBV-transformed lymphoblastoid cell line, HCC1143 BL, from the same patient is available as ATCC

 CRL-2362D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation