Human rotavirus (ATCC® VR-2104)

Classification: Reoviridae, Rotavirus, Rotavirus A  /  Product Format: frozen 1 mL per vial

Classification Reoviridae, Rotavirus, Rotavirus A
Deposited As Human rotavirus, strain Hu/Australia/10-25-10/77L
Agent Human rotavirus
Strain Hu/Australia/102510/77/L
Enteric Research
Biosafety Level 2
Stools of human neonate in a Melbourne maternity hospital in 1977
Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
This item is a Group A rotavirus with serotype: G3P2A[6], showing typical rotavirus morphology by electron microscopy. It has a diameter of 65-75 nm for the double-shelled form and 55-65 nm for the single-shelled form.
Effect on Host
CPE, cell degeneration and sloughing
Recommended Host
MA-104 Clone 1 (ATCC CRL-2378.1)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 98-100% confluent. Calculate volume of virus seed required to inoculate with an optimal MOI (e.g. 0.2-0.4). Prepare a 1:2 dilution of the calculated virus seed amount with Virus Activation Medium [EMEM supplemented with 20 µg/ mL Trypsin (1:250) Powder]. Activate virus for 30 minutes in 37°C water bath. If needed, combine activated virus with enough virus growth medium to inoculate with a small amount of inoculum (e.g. 1 mL per 25 cm2). Remove medium and then rinse cells twice with neat EMEM. Remove EMEM and then inoculate with the prepared inoculum. Adsorb 1.5-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking continuously at a low speed. End adsorption by removing the inoculum and then adding virus growth medium.
Incubation: 1-2 days
Effect on Host
CPE, cell degeneration and sloughing
Name of Depositor G Barnes
Stool sample of a human neonate in a Melbourne maternity hospital, 1977
Year of Origin 1977

Albert MJ, et al. Live attenuated human rotavirus vaccine. US Pat. 4,624,850 dated Nov 25, 1986.

Barnes GL, et al. Early phase II trial of human rotavirus vaccine candidate RV3. Vaccine. (20): 2950-2956, 2002. PubMed: 12126907

Dyall-Smith ML, et al. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 12(9):3973-3982, 1984