Human rhinovirus 31 (ATCC® VR-1795)

Classification: Picornaviridae, Rhinovirus, Human rhinovirus A  /  Product Format: frozen

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Classification Picornaviridae, Rhinovirus, Human rhinovirus A
Agent Human rhinovirus 31
Strain 140 F
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Images VR-1795 Images
Comments This item has been treated with diethyl ether to remove Mycoplasma orale at the ATCC.  The previous item number was VR-506™
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
HeLa (ATCC® CCL-2™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.005 - 0.01 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor VV Hamparian and RM Conant
Source Human throat washings
References

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. I. HeLa cells for propagation and serologic procedures. J Immunol 100 (1): 107-113, 1968. PubMed: 4295059

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. II. Serologic characterization of prototype strains. J Immunol 100 (1): 114-119, 1968. PubMed: 4295060

Connelly AP Jr., Hamre D. Virologic studies on acture respiratory disease in young adults. II. Characteristics and serolotic studies of three new Rhinoviruses. J. Lab. Clin. Med. 63: 30-43, 1964. PubMed: 14102901

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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