Influenza B virus (ATCC® VR-1784)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: 1 mL per vial

Classification Orthomyxoviridae, Influenzavirus B
Agent Influenza B virus
Strain B/Virginia/ATCC4/2009
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1784 images
Comments This item was isolated in cell culture by four passages in MDCK cells.
Effect on Host CPE, cell rounding and sloughing
Recommended Host MDCK (ATCC® CCL-34™)
Growth Conditions Temperature: 35°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 2-3 days
Effect on Host CPE, cell rounding and sloughing
Name of Depositor ATCC
Source Specimen from a clinical patient in Virginia, 2009