Sandfly fever sicilian virus (ATCC® VR-1756)

Classification: Bunyaviridae, Phlebovirus  /  Product Format: frozen 1 mL per vial

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Classification Bunyaviridae, Phlebovirus
Deposited As Phlebotomus virus (Sicily)
Agent Sandfly fever sicilian virus
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, rounding and sloughing
Recommended Host
BHK-21 [C-13] (ATCC® CCL-10™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 30-50% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium
Incubation: 4-5 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor SE Grossberg, Medical College of Wisconsin
Chain of Custody ATCC <-- SE Grossberg, Medical College of Wisconsin
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation