A717 (ATCC® HB-9596)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for glycated human serum albumin.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycated serum albumin (glycoalbumin)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with native glycated human serum albumin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for glycated human serum albumin.
It does not react with unglycated albumin.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Medium Renewal: Every 2 to 3 days
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG , IgG
    Name of Depositor Exocell, Inc.
    References

    Cohen MP. Treatment of complications of diabetes with substances reactive with the fructosyl-lysine structure in glycated albumin. US Patent 5,518,720 dated May 21 1996

    Cohen MP, Hud E. Production and characterization of monoclonal antibodies against human glycoalbumin. J. Immunol. Methods 117: 121-129, 1989. PubMed: 2913157

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Cohen MP. Treatment of complications of diabetes with substances reactive with the fructosyl-lysine structure in glycated albumin. US Patent 5,518,720 dated May 21 1996

    Cohen MP, Hud E. Production and characterization of monoclonal antibodies against human glycoalbumin. J. Immunol. Methods 117: 121-129, 1989. PubMed: 2913157