Frozen cultures arrayed in seventy-four 96-well plates.
Strains were derived from parent strains BY4730 (ATCC 200892)
(leu2delta0 met15delta0 ura3delta0) and BY4741 (ATCC 201388)
(his3delta1 leu2delta0 met15delta0 ura3delta0). Deletion strains were generated using a PCR-based gene deletion strategy. Each ORF disruption was made by replacing the ORF with a KanMX module and uniquely tagged with one or two 20mer sequence(s).