K-562-GFP (ATCC® CCL-243-GFP)

Organism: Homo sapiens, human  /  Tissue: bone marrow  /  Disease: chronic myelogenous leukemia, CML

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Organism Homo sapiens, human
Tissue bone marrow
Product Format frozen 1.0 mL
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain CMV and HPV viral sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease chronic myelogenous leukemia, CML
Age 53 years
Gender female
Ethnicity NA
Applications The K-562 cell line has attained widespread use as a highly sensitive in vitro target in the natural killer cell cytotoxicity assay. High level expression of GFP by CCL-243-GFP enables highly accurate quantitation of killing by flow cytometry.
Storage Conditions liquid nitrogen vapor phase
Karyotype

68-69,XX,-X,add(1)(p13),add(1)(p36.1),add(2)(q33),

-3,dup(6)(p21.1p23),+7,add(7)(p11.2),

-9,del(9)(p13),der(9)t(9;9)(p13;q22),der(10)t(3;10)(p13;q22),-13,add(13)(p10),

-14,add(17)(p11.2)x2,der(18)t(1;18)(p32;q23),-20,-21,-22,

+mar1,+mar2,+mar3,+mar4,+mar5,+mar6,+mar7[cp10]
Images
Comments Derived from parental line CCL-243, Lot 61978333. Stably expresses GFP under the control of the human EF-1 alpha promoter utilizing puromycin as the antibiotic selection agent. During routine culture puromycin (0.5 µg/mL) should be added directly to the cells in culture (during initial seeding and subculture).
Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium (IMDM; ATCC® 30-3005™). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (FBS; ATCC® 30-2020™) to a final concentration of 10% and puromycin to a final concentration of 0.5 µg/mL.

Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium with no FBS added, supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial 5 x 106 vialed, with a yield of ≥ 2 x 106 viable cells upon thaw
Volume 1.0 mL
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Population Doubling Time 18 ± 2 hours
Name of Depositor ATCC Cell Systems
Year of Origin 2016
Cross References

Nucleotide (GenBank) : X96391 H.sapiens mRNA for alternatively spliced isoform of CLC-6 chloride channel.

Nucleotide (GenBank) : AF146430 Homo sapiens cell-line K562 sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation