p425 GAL1 (ATCC® 87331)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Designations p425 GAL1
Permits and Restrictions

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Depositors M Funk
Biosafety Level 1
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.5399999618530270
Intact vector size: 7.540
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS425, GAL1 promoter
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...SpeI, ->, 3388-3445
promoter for expression: GAL1, <-, 3452-3912
marker(s): ampR, <-, 5063-5993
replicon: 2 micron, ->, 6093-7433
Vector: p425 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS425, GAL1 promoter
Construct size (kb): 7.539999961853027
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9 (doublet); EcoRI--5.1, 2.4; KpnI--5.8, 1.7. It appears that the SacI site at the start of the GAL1 promoter has been lost.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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