Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 700792-FZ)

Strain Designations: 14797-2  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations 14797-2
stool of a 13-year-old female with fulminant hepatitis and severe encephalopathy, Michigan, 1977
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information Frozen
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Preparation of Clostridium difficile strain 14797-2 ATCC® 700792-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 005
Presence of tcdA and tcdB genes confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
This strain was isolated from what may possibly be the first reported case of Clostridium difficile-associated diarrhea (CDAD).
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture 80% N2 -10% CO2 -10% H2
Genotype Toxinotype: 0
Name of Depositor FR Fekety
Special Collection NCRR Contract
stool of a 13-year-old female with fulminant hepatitis and severe encephalopathy, Michigan, 1977
Year of Origin 1977

Fekety R, et al. The hunt for Clostridium difficile: 21-year follow-up of a stool specimen sent for culture. Lancet 354: 1115-1116, 1999. PubMed: 10509517

F. R Fekety, personal communication

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986