RK2 (ATCC® 37125)

Applications: shuttle vector  /  Depositors: DR Helinski

Permits and Restrictions

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Designations RK2
Depositors DR Helinski
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 56.0000000000000000
Vector: RK2 (plasmid)
Construction: natural isolate
Construct size (kb): 56.0
Features: marker(s): ampR, kanR, tetR
replicon: RK2
shuttle vector
natural isolate from Pseudomonas sp.
contains relaxation complex site (rlx).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Meyer R, et al. Molecular vehicle properties of the broad host range plasmid RK2. Science 190: 1226-1228, 1975. PubMed: 1060178

Ditta G, et al. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347-7351, 1980. PubMed: 7012838

Shipped freeze-dried
Shipping Information Distributed: freeze-dried