Size (kb): 10.6669998168945300
Vector: YEp13 (plasmid)
Promoters: Promoter none
Construction: pBR322, Saccharomyces cerevisiae LEU2, 2 micron ori
Construct size (kb): 10.66699981689453
Features: marker(s): LEU2
replicon: 2 micron
YE-type (episomal) shuttle vector
vector for constructing genomic libraries
Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7.
Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA.
YEp13 is mitotically stable in cir+ strains.
A YE-type S. cerevisiae/E. coli episomal shuttle vector.
An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 was cloned into the 2 micron PstI site.
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Shipped: Frozen glycerol stock of Escherichia coli containing the plasmid.