YEp13 (ATCC® 37115)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: JR Broach

Designations YEp13
Depositors JR Broach
Biosafety Level 1
Vector Information
Size (kb): 10.6669998168945300
Vector: YEp13 (plasmid)
Promoters: Promoter none
Construction: pBR322, Saccharomyces cerevisiae LEU2, 2 micron ori
Marker(s):LEU2,ampR,tetR
Construct size (kb): 10.66699981689453
Features: marker(s): LEU2
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7.
Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA.
YEp13 is mitotically stable in cir+ strains.
A YE-type S. cerevisiae/E. coli episomal shuttle vector.
An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 was cloned into the 2 micron PstI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

Related Products
component of:ATCC 87635
Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli containing the plasmid.

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