Chlorella vulgaris Beijerinck (ATCC® 30821)

Depositor: MP Starr  /  Biosafety Level: 1

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Biofuel production
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

reservoir, Ukraine, USSR, 1966(?) Host for Vampirovibrio chlorellavorus ATCC 29753
Product Format frozen
Type Strain no
Prey-predator interaction
Medium ATCC® Medium 5: Sporulation agar
Growth Conditions
Temperature: 25.0°C
Duration: axenic; under 150 foot candles

1.   Harvest cells from a culture which is at or near peak density by adding 3.0 ml fresh ATCC medium 5 broth to the slant and washing cells into suspension.

2.   Adjust the concentration of cells to 2 x 106 -2 x 10/ ml with fresh broth medium, then dilute to half this concentration by adding an equal amount of a 10% (v/v) sterile methanol solution in fresh ATCC medium 5 broth.

3.  Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.

4.   Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)   

5.   The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.

6.   To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.

7.   Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 ml of ATCC medium 5 without agar.  Centrifuge at 300 x g for 5 min.

10.Remove most of the supernatant (=methanol, which can inhibit growth) and then resuspend the pellet.  Transfer the culture to a fresh tube of ATCC medium 5 and incubate on a 15° horizontal slant at 50-100 µEinsteins/m2/s irradiance at 25°C with the cap loosened one half turn.  Maintain under a 14/10 h light-dark photoperiod and subculture every 14-21 days.

Name of Depositor MP Starr
Chain of Custody
ATCC <<--MP Starr<<--ICPB 4049 B.V. Gromov <<--- K.A. Mamkaeka
Year of Origin 1966

Coder DM, Starr M. Antagonistic association of the chlorellavorus bacterium ('Bdellovibrio' chlorellavorus) with Chlorella vulgaris. Curr. Microbiol. 1: 59-64, 1978.

Mikrobiologiya 35: 853-859, 1966.

Tsitologiya 14: 256-260, 1976.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation