ITS Solution (Insulin, Transferrin, Selenious Acid) (ATCC® 30-2606)

Permits and Restrictions

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Product Format 10 mL
Storage Conditions Store medium at 2°C to 8°C. Do not freeze.

Insulin, Transferrin and Selenious Acid (ITS) have been demonstrated to improve performance in serum-free media. Insulin promotes the uptake of glucose and amino acids. Transferrin is an iron-transport protein, and with selenious acid, supplies essential trace elements normally provided by serum. ITS Solution is free of animal origin.


Supplied at a 100x concentration. For use, aseptically add 5 mL of ITS solution per 500 mL of medium.

Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot using current USP methods. pH, osmolality, and mycoplasma tests are also performed as indicated below.




2.6 to 3.4


2 to 6 mOsm/kg H2O

Sterility Testing




*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation