Mammary Epithelial Cell Growth Kit (ATCC® PCS-600-040)

Permits and Restrictions

View Permits

Biosafety Level N/A
Product Format frozen kit
Storage Conditions -20°C (or -70°C for long-term storage)
Comments Mammary Epithelial Cell Growth Kit contains all components to be added to Mammary Epithelial Cell Basal Medium (ATCC PCS-600-030) to create a complete medium designed to support epithelial cells derived from normal human female breast.  Mammary Epithelial Cell growth kit contains rH-Insulin, L-Glutamine, Epinephrine, Apo-Transferrin, rH-TGFα, Extract P, and Hydrocortisone Hemisuccinate.

Component

Final Concentration

rH-Insulin

5 µg/mL

L-Glutamine

6mM

Epinephrine

1 µM

Apo-Transferrin

5 µg/mL

rH-TGF-α

5 ng/mL

ExtractP

0.4%

Hydrocortisone Hemmisuccinate

100 ng/mL

Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the Mammary Epithelial Cell Growth Kit (PCS-600-040) just prior to adding them to the basal medium.
  3. Obtain one bottle of Mammary Epithelial Cell Basal Medium (PCS-600-030, 485 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each transfer.

  6. Component

    Volume

    Final Concentration

    rH-Insulin

    0.5 mL

    5 µg/ mL

    L-Glutamine

    15 mL

    6 mM

    Epinephrine

    0.5 mL

    1 µM

    Apo-Transferrin

    0.5 mL

    5 µg/ mL

    rH-TGF-α

    0.5 mL

    5 ng/ mL

    ExtractP

    2 mL

    0.4%

    Hydrocortisone Hemmisuccinate

    0.5 mL

    100 ng/ mL

     

  1. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  2. Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.
Volume kit
Sterility Tests negative for bacteria, fungi and yeast
Functional Tests rate of proliferation and morphology
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation