Small Airway Epithelial Cell Growth Kit contains components that when added to Airway Epithelial Cell Basal Medium (ATCC PCS-300-030) create a complete ATCC® Primary Cell Solutions™ culture environment for small airway epithelial cells derived from normal human lung. The serum-free medium formulation is designed to support normal airway epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.
The final concentration for each component in complete small airway epithelial cell growth medium is as follows:
- L-alanyl-L-glutamine: 2.4 mM
- Extract P: 0.4%
- Plasma Protein Fraction: 0.5%
- Airway Epithelial Cell Supplement: Epinephrine 1.0 µM, Transferrin 5 µg/ml, T3 10 nM, Hydrocortisone 5 µg/ml, rh EGF 5 ng/ml, rh Insulin 5 µg/ml