pGRN145 plasmid in E. coli GC10 (ATCC® MBA-141)

Depositors: Geron Corporation

Permits and Restrictions

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Designations pGRN145 plasmid in E. coli GC10
Depositors Geron Corporation
Applications Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit)
Total size (kb): 14.002
Bacterial selection marker: cmlR


hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
MPSV: 3643-4119 (promoter)
Ori: 12165-12747


Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.

Gene: Telomerase reverse transcriptase (TERT)
Source: Human
Insert size (kb): 3.398
GenBank accession: NM_003219
Media ATCC® Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen glycerol stock of E. coli GC10 containing the plasmid
Bascic Documentation

This material is subject to claims under U.S. Patent Nos. 6,261,836 and 6,337,200, other pending patent applications, and foreign counterparts thereof. It is required that the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment.  Commercial and For Profit Organizations need to obtain a license from Geron Corporation by contacting before shipment.

Attachments MSDS
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