• remove Product Category: Stem Cells

Specialty Cell Culture Media

  • Neural Progenitor Cell Dopaminergic Differentiation Kit (ATCC® ACS-3004)

    ATCC® Number: ACS-3004

    Applications:

    The media is specially designed for the dopaminergic differentiation of iPSC-derived neural progenitor cells (NPCs).

    Complete dopaminergic differentiation medium for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3004). To make complete NPC medium add the following components of this kit to 250 mL DMEM: F12:

    237 mL DMEM: F12 (Basal medium)
    2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL  Dopa Differentiation Kit Component C

    For-Profit:   $315.00 Non-Profit:   $315.00

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    Product Format: frozen
    Comments: 2.5 mL L-Alanyl-L-Glutamine
    2.5 mL Non-Essential Amino Acids + 0.5 mL Ascrobic Acid
    5 mL Dopa Differentiation Kit Component A
    2.5 mLDopa Differentiation Kit Component B
    0.5 mL Dopa Differentiation Kit Component C
  • Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003)

    ATCC® Number: ACS-3003

    Storage: -20°C
    Applications:

    The media is specially designed for expansion of iPSC-derived neural progenitor cells (NPCs).

    Complete growth media for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of this kit to 464 mL DMEM: F12:

    5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D

    For-Profit:   $310.00 Non-Profit:   $310.00

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    Product Format: frozen
    Comments: 5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D
    Focus Area:

    Neuronal Differentiation And Drug Screening

  • Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041)

    ATCC® Number: PCS-500-041

    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)

    For-Profit:   $120.00 Non-Profit:   $120.00

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    Product Format: frozen 1 kit
    Comments:

    The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:

    • rh FGF basic: 125 pg/mL
    • rh IGF-1: 15 ng/mL 
    • Fetal Bovine Serum: 7% 
    • L-Alanyl-L-Glutamine: 2.4 mM   
  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011

    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.50 Non-Profit:   $25.50

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  • For-Profit:   $699.00 Non-Profit:   $699.00

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  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010

    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $92.00 Non-Profit:   $92.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020

    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $51.00 Non-Profit:   $51.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Stem Cell Freezing Media (ATCC® ACS-3020-3)

    ATCC® Number: ACS-3020-3

    Quantity: 3 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $128.00 Non-Profit:   $128.00

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    Product Format: frozen 3 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002

    Quantity: 500 mL
    Storage: Complete medium is stored at -20°C

    For-Profit:   $235.00 Non-Profit:   $235.00

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    Product Format: frozen 500 mL
    Comments:
    This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
  • ROCK Inhibitor Y27632 (ATCC® ACS-3030)

    ATCC® Number: ACS-3030

    Quantity: 10 mg
    Storage: -20°C to -80°C

    For-Profit:   $199.00 Non-Profit:   $199.00

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    Product Format: dried 10 mg
    Comments:
    Purity is > 98% as confirmed by HPLC analysis. Structure and mass are confirmed by NMR and mass spectrometry.
    Reconstituted product can be aliquoted and stored at -20°C for up to six months. Protect from light.