C58(NT)D.1.G.OUAR.1 (ATCC® TIB-236)

Organism: Rattus norvegicus, rat  /  Cell Type: T lymphocyte  /  Disease: lymphoma

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Organism Rattus norvegicus, rat
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain WFu
Applications
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Storage Conditions liquid nitrogen vapor temperature
Comments
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor R Hyman
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480

Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480

Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188