AKR1.G.1.OUAR.1.26 (ATCC® TIB-232™) Organism: Mus musculus, mouse / Tissue: thymus / Disease: lymphoma General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Tissue thymus Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease lymphoma Strain AKR/J Applications The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.The line may be useful for fusion studies and as a host for the transfection of cloned genes. Antigen Expression Thy-1; L3T4; Lyt-2; HSA Genes Expressed Thy-1; L3T4; Lyt-2; HSA Comments The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.The line may be useful for fusion studies and as a host for the transfection of cloned genes.The cells have the surface antigen properties of cortical thymocytes. Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Name of Depositor R Hyman Deposited As Mus musculus References . . Immunogenetics 10: 261-271, 1980. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS References . . Immunogenetics 10: 261-271, 1980.