S194/5.XXO.BU.1 (ATCC® TIB-20)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1
Comments
Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgA (not secreted); kappa light chain (not secreted)
Name of Depositor R Hyman, P Ralph, S Sarkar
References

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Basic Documentation
References

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881