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Complete Growth Medium
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The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing
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Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 105 viable cells/mL.
Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
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Cryopreservation
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culture medium 95%; DMSO, 5%
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Culture Conditions
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Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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