FO ATCC ® CRL-1646™ Mus musculus plasmacytoma; myeloma

Permits and Restrictions	View Permits
Organism	Mus musculus, mouse
Cell Type	B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Strain	BALB/c
Applications	The line can be used as a fusion partner for making B cell hybridomas.

Derivation	The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
Antigen Expression	H-2d
Comments	The cells are resistant to 8-azaguanine and do not produce immunoglobulin. Tested and found negative for ectromelia virus (mousepox).

Derivation

The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.

Antigen Expression

H-2d

Comments

The cells are resistant to 8-azaguanine and do not produce immunoglobulin.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10⁵ viable cells/mL. Maintain cell density between 1 X 10⁵ and 1 X 10⁶ viable cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Population Doubling Time	8 to 10 hrs

Name of Depositor	SF de St. Groth
References	de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
References	de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747

FO (ATCC^® CRL-1646^™)