P3X63Ag8U.1 (ATCC® CRL-1597)

Organism: Mus musculus, mouse  /  Cell Type: B lymphoblast  /  Disease: plasmacytoma; myeloma

Organism Mus musculus, mouse
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Gender female
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was derived from the MOPC 21 - P3X63Ag8 lineage.
Clinical Data
female
Antigen Expression
H-2d
Comments
The cells do not synthesize gamma 1 chains.
They produce but do not secrete kappa chains.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 x 105 viable cells/mL and maintain below 1 x 106 cells/mL.
Medium Renewal: 3 times per week
Cryopreservation

Freeze medium: Culture medium, 95%; DMSO, 5%.

Culture Conditions
Temperature: 37°C
Name of Depositor MD Scharff
References

Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551

Cross References

Nucleotide (GenBank) : E02974 DNA encoding mouse interleukin-6(IL-6) receptor.

Basic Documentation
References

Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551