BW5147.G.1.4.OUAR.1 (ATCC® CRL-1588)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain AKR/J
Applications
They can be used to create T cell hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2k
Genes Expressed
H-2k
Comments
The cells are ouabain resistant.
They can be used to create T cell hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp4 viable cells/ml. Subculture every 3 to 4 days.
Name of Depositor R Hyman
References

Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550

Basic Documentation
References

Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550