C166-GFP (ATCC® CRL-2583)

Organism: Mus musculus, mouse  /  Cell Type: endothelial  /  Tissue: Yolk sac  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue Yolk sac
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2
[Cells contain SV40 and HSV viral sequences]
Age 12 day embryo
Storage Conditions liquid nitrogen vapor phase
Derivation
The C166-GFP cell line was derived from the C166 cell line (ATCC CRL-2581) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).

The C166 cell line was established from cells from F1 embryos obtained by mating a female NMRI/GSF mouse with a male CD-1 mouse that was transgenic for the human fes (fps/fes) proto-oncogene.

Comments
The vector contains cytomegalovirus (CMV), SV40 and Herpes simplex virus (HSV) viral DNA sequences and the neomycin resistance gene.

These mice express multiple copies of an activated allele of the human fes (fps/fes) proto-oncogene and display hypervascularity progressing to multifocal hemangiomas.

For additional details see C166 (ATCC CRL-2581).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 0.2 mg/ml G -418
  • fetal bovine serum to a final concentration of 10%
  • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze Medium: Complete growth medium 95%; DMSO, 5%
    Storage Temperature: Liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Name of Depositor R Auerbach
    References

    Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159

    Lu LS, et al. In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line. Proc. Natl. Acad. Sci. USA 93: 14782-14787, 1996. PubMed: 8962132

    Basic Documentation
    Other Documentation
    Restrictions

    This product's use is governed by the Limited Use License. For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, contact the Licensing Department, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, California 92008. Phone (760) 603-7200 or outlicensing@lifetech.com

    References

    Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159

    Lu LS, et al. In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line. Proc. Natl. Acad. Sci. USA 93: 14782-14787, 1996. PubMed: 8962132