YIp5-KanR (ATCC® 37473)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: CA Reddy

Permits and Restrictions

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Designations YIp5-KanR
Depositors CA Reddy
Biosafety Level 1
Host
Shipped in: E. coli C600
Vector Information
Size (kb): 7.0999999046325680
Vector: YIp5-KanR (plasmid)
Construction: kanR of pNG35 into PvuII site of YIp5
Marker(s):URA3,ampR,kanR,tetR
Construct size (kb): 7.099999904632568
Features: marker(s): ampR, kanR, tetR, URA3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
Vector provides selection markers for transformation of Saccharomyces cerevisiae or other eukaryotes based on G418 resistance.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rao TR, Reddy CA. A YIp5-kanR plasmid useful for isolating ars from yeast and other eukaryotes based on G418 resistance selection. Nucleic Acids Res. 14: 7504, 1986. PubMed: 3532035

Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet