DNA from Vaccinia virus (TC-adapted), strain WR [ATCC® VR-1354™] (ATCC® VR-1354D)

Organism: Vaccinia virus  / 

Product Format frozen
100 µL per vial, shipped frozen
Storage Conditions -70°C or colder
Applications
It is suitable for use in PCR and other molecular virology applications.
Comments
Viral nucleic acids, including virus genomic DNA, in total DNA isolated from BS-C-1 (ATCC® CCL-26™) infected with Vaccinia virus (TC-adapted), strain WR. This product was prepared using methods known to inactivate viruses. It is suitable for use in PCR and other molecular virology applications.

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Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications
  1. Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
  2. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µL of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.
  3. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
  4. Identity is confirmed by sequencing of approximately 300 bp to 1000 bp of the PCR amplicon.