Synthetic West Nile Virus RNA (ATCC® VR-3198SD)

Product Format: 1 vial/100 reactions

Strain Synthetic West Nile Virus RNA
Applications The Synthetic West Nile Virus RNA contains single stranded RNA genetic material designed and synthetically created for use as a genetic surrogate for West Nile Virus, known as molecular standards. Molecular standards are appropriate for use as control material in molecular applications, such as PCR.
Biosafety Level 1
Product Format 1 vial/100 reactions
Storage Conditions 8°C or colder
Comments

Genetic Target: Fragments from the 5'UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3' UTR region of the West Nile Virus genome

Length: ~1500 bp

Format Provided: Dried RNA  

Starting Amount: 1.1 x 108 Copy Numbers (CN)  

Downstream compatibility: Reverse Transcription PCR or other molecular applications  

Special Collection DNA
References

Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368

Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934

Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613

Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069

Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997

Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762

Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039

Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227

Basic Documentation
FAQs
  1. Stability of Norovirus and West Nile standards

    Accelerated stability studies at 45oC have been performed on these samples and no degradation was observed.


    Date Updated: 3/27/2014