Murine hepatitis virus (ATCC® VR-765)

Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2  /  Product Format: frozen

Classification Nidovirales, Coronaviridae, Coronavirus,Group 2
Deposited As Mouse hepatitis virus
Agent Murine hepatitis virus
Strain MHV-JHM
Common Name Murine hepatitis virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
tissue, animal
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Effect on Host
Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells
CPE, fusing and clumping cells, cells floating
Recommended Host

Recommended Host: NCTC clone 1469 cells (ATCC® CCL-9.1™)   

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Temperature: 37°C
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells
CPE, fusing and clumping cells, cells floating
Name of Depositor J Parker
Special Collection ATCC
Source
tissue, animal
References

Cheever FS, et al. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. J. Exp. Med. 90: 181-194, 1949.

or colder

brain of 17-day-old Swiss mouse with spontaneous flaccid paralysis of hind legs

or colder