Organism: Beet curly top virus  /  Clone Type: Clone  /  Depositors: DC Stenger

Permits and Restrictions

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Designations pLOGAN
Species Beet curly top virus
Depositors DC Stenger
contains sequence
Plant research
Construct size (kb): 5.73

Construction of the clone:  Plasmid constructed by inserting 3038 bp Sal I linearized viral dsDNA into Sal I site of pUC8vector.

Insert lengths(kb): 3.04
Tissue: Beet curly top virus
Insert Size (kb) 3.038
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Plasmid constructed by inserting 3038 bp SalI linearized viral dsDNA into SalI site of pUC8 vector. >95% sequence identity wiith BCTV- California. The clone is infectious. The ATCC sample contains 30ug of purifed plasmid DNA in TE buffer.
Classification Curtovirus

Stenger DC, et al. Genomic characterization of phenotypic variants of beet curly top virus. J. Gen. Virol. 71: 2211-2215, 1990. PubMed: 2230726

Stenger DC. Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol. Plant-Microbe Interact. 7: 154-157, 1994. PubMed: 8167369

Stenger DC, et al. Phylogenetic relationships within the family Potyvirdae: wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology 88: 782-787, 1998.