pSP98 (ATCC® PVMC-25)

Organism: tomato yellow leaf curl bigeminivirus  /  Clone Type: Clone  /  Depositors: E Moriones

Permits and Restrictions

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Designations pSP98
GenBank Number


Species tomato yellow leaf curl bigeminivirus
Depositors E Moriones
contains sequence
Plant research
Construct size (kb): 15.0

Construction of the clone:  A 1.8mer partial tandem repeat of the genome of M-TYLCV-Sar into binary vector pBin19.

Name of vector: Bin 19
Intact vector size: 10.000
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: BamHI EcoRI HindIII
Polylinker sites: BamHI EcoRI HindIII SmaI SalI KpnI XbaI SstI
Construction: pRK252, pTiT7, pTiT37
Host range: Agrobacterium tumefaciens; higher plants; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
replicon: RK2
Cross references:
DNA: genomic
Insert lengths(kb): 2.700000047683716
Insert Size (kb) 2.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Classification Geminivirus

Noris E, et al. High similarity among the tomato yellow leaf curl virus isolates from the west Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. Arch. Virol. 135: 165-170, 1994. PubMed: 8198442