pOT2 (ATCC® PVMC-1)

Organism: Odontoglossum ringspot virus  /  Clone Type: Clone  /  Depositors: SM Wong

Designations pOT2
Species Odontoglossum ringspot virus
Depositors SM Wong
Applications
contains sequence
Plant research
Vector
Construct size (kb): 6.0

Plasmid constructed by inserting full-length cDNA clone of ORSV into pBlueScript  plasmid

Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: Cattleya odontoglossum ringspot
Disease: odontoglossum ringspot
Gene product: [ORSV clone]
Insert Size (kb) 3.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Highly infectious in vitro RNA transcripts can be obtained using Promega's In Vitro Transcription kit (T7 RNA polymerase).
Plasmid constructed by inserting full-length cDNA clone of odontoglossum ringspot tobamovirus (ORSV) into pBluescript KS+ vector.
Classification Tobamovirus
References

Yu HH, Wong SM. A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene. Arch. Virol. 143: 163-171, 1998. PubMed: 9505974