Influenza A virus (H1N1) (ATCC® VR-1736)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/Virginia/ATCC1/2009
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Isolation
This item was isolated in tissue culture.
Product Format frozen
Storage Conditions   -70°C or colder
Comments
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Effect on Host
CPE, rounded refractile cells with cell degeneration
 CPE, rounded refractile cells with cell degeneration.
Recommended Host
MDCK (ATCC CCL-34)

Production Host:  MDCK (ATCC® CCL-34™)

Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with virus growth medium to provide a MOI 0.1. Adsorb 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Grow item in serum free media that contains 2.0 μg/mL TPCK-treated trypsin, 0.125% BSA Fraction V and 10mM HEPES Buffer.
Incubation: 2-5 days
Effect on Host
CPE, rounded refractile cells with cell degeneration
 CPE, rounded refractile cells with cell degeneration.
Name of Depositor ATCC®
Special Collection ATCC
Source
Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation