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Influenza A virus (H3N2) (ATCC® VR-1679™)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Hong Kong/8/68
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1679 images
Comments
Derived by adaptation from allantoic product ATCC® VR-544™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-544™ and ATCC® VR-1679™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture.
Effect on Host
CPE, rounding and degeneration
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and degeneration
Name of Depositor MT Coleman, Centers of Disease Control and Prevention; ATCC®
Source Isolated from a patient in Hong Kong, 1968
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

. . WHO Weekly Epid. Record 43: 401, 1968.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
Micrograph of Host Effects