Chlamydia trachomatis (ATCC® VR-903)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen

Classification Chlamydiaceae, Chlamydia
Deposited As Lymphogranuloma venereum type III
Agent Chlamydia trachomatis
Strain Lymphogranuloma venereum (LGV III) strain 404
Applications
Sexually Transmitted Disease Research
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Effect on Host
CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA. Death of chicken embryo.
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions

Recommendations for Infection: For best results in tissue culture, cells should be 24 to 48 hours old and 80% - 100% confluent. The cells will not continue to grow after adding the cycloheximide.

Incubation:  In TC, 2 to 3 days at 37°C, a 5% CO2 in air atmosphere is recommended. In CE, 7 to 10 days at 35°C.

Effect on Host
CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA. Death of chicken embryo.
Name of Depositor J Schachter
Source Lymph node from human with LGV
References

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529