Chlamydia trachomatis Serovar D (ATCC® VR-885)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydia
Deposited As Trachoma serotype d
Agent Chlamydia trachomatis Serovar D
Strain Trachoma type D strain UW-3/Cx
Sexually Transmitted Disease Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Rapid loss in titer when stored above -70°C.

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL Cycloheximide.

Effect on Host
CPE, intracellular inclusion bodies
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, 5% CO2 in air atmosphere is recommended
Effect on Host
CPE, intracellular inclusion bodies
Name of Depositor SP Wang
Source Human cervix, asymptomatic

Alexander ER. . Am. J. Opthalmol. 63: 1469, 1967.

Holt S. Am. J. Opthalmol. 63: 1057, 1967.

Wang SP. Am. J. Opthalmol. 70: 367, 1970.