P22 (ATCC® 97541)

Strain Designations: r34  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations r34
Application
Potential bacteremia therapeutic agent
This is one of six bacteriophage strains deposited at the ATCC (97537-97539 are lambda strains and 97540-97542 are P22 strains) that remain in the mouse circulatory system for long periods of time. They are potential therapeutic agents for bacteremias.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol® no
Type Strain no
Comments
Recommendation for recovery of bacteriophage is to adsorb lysate to overnight grown S. Typhimurium LT2 at 37C for 18 minutes, plate adsorbtion mixture onto LB agar plates, and then incubate overnight at 37C.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor National Institutes of Health/NIMH, CR Merril, National Institutes of Health/NIMH
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
References

Merril CR, et al. Long-circulating bacteriophage as antibacterial agents. Proc. Natl. Acad. Sci. USA 93: 3188-3192, 1996. PubMed: 8622911

wild-type