Escherichia coli bacteriophage Lambda Argo1 (ATCC® 97539)

Strain Designations: Argo1  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations Argo1
Host Organism
Escherichia coli strain MG1655 (ATCC 47076)
Application
Potential bacteremia therapeutic agent
This is one of six bacteriophage strains deposited at the ATCC (ATCC® 97537™-97539™ are lambda strains and ATCC® 97540™-97542™ are P22 strains) that remain in the mouse circulatory system for long periods of time. They are potential therapeutic agents for bacteremias.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Shipping Information Distributed: freeze-dried bacteria-free lysate
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Comments
There is a G->A transition at nucleotide 6606 of the lambda capsid E major head protein gene in Argo1. This results in a lysine(basic) to glutamate(acidic) substitution.
To recover bacteriophage, adsorb lysate for 18 minutes at 37°C to an overnight culture of Escherichia coli MG1655. Plate the adsorbtion mixture onto LB agar plates, and then incubate overnight at 37°C.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor National Institutes of Health/NIMH, CR Merril, National Institutes of Health/NIMH
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Merril CR, et al. Long-circulating bacteriophage as antibacterial agents. Proc. Natl. Acad. Sci. USA 93: 3188-3192, 1996. PubMed: 8622911