bacteriophage lambda (ATCC® 97539)

Strain Designations: Argo1  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations Argo1
Application
Potential bacteremia therapeutic agent
This is one of six bacteriophage strains deposited at the ATCC (97537-97539 are lambda strains and 97540-97542 are P22 strains) that remain in the mouse circulatory system for long periods of time. They are potential therapeutic agents for bacteremias.
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Shipping Information Distributed: freeze-dried bacteria-free lysate
Preceptrol® no
Type Strain no
Comments
There is a G->A transition at nucleotide 6606 of the lambda capsid E major head protein gene in Argo1. This results in a lysine(basic) to glutamate(acidic) substitution.
To recover bacteriophage, adsorb lysate for 18 minutes at 37C to an overnight culture of Escherichia coli MG1655. Plate the adsorbtion mixture onto LB agar plates, and then incubate overnight at 37C.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor National Institutes of Health/NIMH, CR Merril, National Institutes of Health/NIMH
References

Merril CR, et al. Long-circulating bacteriophage as antibacterial agents. Proc. Natl. Acad. Sci. USA 93: 3188-3192, 1996. PubMed: 8622911