Monoclonal antibody to West Nile virus Envelope Protein, clone E18 (produced in vitro) (ATCC® VR-1611)

Organism: Monoclonal antibody to West Nile virus Envelope Protein, clone E18 (produced in vitro)  / 

Focus Area
Infectious diseases
Product Format frozen
Each vial of ATCC® VR-1611™ contains approximately 100 µL of purified monoclonal antibody in PBS. The concentration, expressed as mg per mL, is shown on the Certificate of Analysis.
Storage Conditions Store at -20°C or colder immediately upon arrival. Repeated freeze-thaw cycles should be avoided.
Applications
Emerging infectious disease research
Comments
Antibody Class: IgG2aκ

The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E18 antibody is reported to bind to the fusion loop of domain II in the envelope glycoprotein.

Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC® CCL-81™) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody has been reported to be non-neutralizing, or weakly neutralizing and reactive using flow cytometry and Western blot analysis.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications
  1. Concentration of antibody is expressed in mg/mL by UV spectrophotometry (absorbance at 280 nm).
  2. Integrity and purity are confirmed by capillary electrophoresis, by observation of  ≥ 80% of total protein distributed between two protein peaks of molecular weight consistent with antibody heavy and light chains.
  3. Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC® CCL-81™) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)].  The antibody has been reported to be non-neutralizing,1 or weakly neutralizing2 and reactive using flow cytometry and Western blot analysis.3
  4. Isotype analysis is performed to verify antibody class, subclass and light chain.