Gallid herpesvirus 2 (ATCC® VR-987)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Permits and Restrictions

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Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 1
Agent Gallid herpesvirus 2
Strain Md-5
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Frozen infected cells should be thawed quickly in 37C water bath and immediately diluted in 3-5 volumes of fresh medium. After the cells attach they should be fed with fresh medium.
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Recommended Host
duck embryo fibroblasts

Production Host:  DEF

chicken; chicken kidney cells; duck embryo fibroblasts
Growth Conditions
Temperature: 38.0°C
Duration: 7 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Name of Depositor RL Witter
Source
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
References

Witter RL. . Avian Dis. 24: 210-232, 1980.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation