Gallid herpesvirus 2 (ATCC® VR-987)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 1
Agent Gallid herpesvirus 2
Strain Md-5
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Frozen infected cells should be thawed quickly in 37C water bath and immediately diluted in 3-5 volumes of fresh medium. After the cells attach they should be fed with fresh medium.
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Recommended Host
duck embryo fibroblasts

Production Host:  DEF

chicken; chicken kidney cells; duck embryo fibroblasts
Growth Conditions
Temperature: 38.0°C
Duration: 7 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Name of Depositor RL Witter
Source
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
References

Witter RL. . Avian Dis. 24: 210-232, 1980.